Capstone projects are projects that senior NYU Abu Dhabi students complete in their respective fields of study. Under the supervision of Prof. Magzoub and postdoctoral researchers, students in our lab learn to design and conduct experiments to answer a research question pertaining to either drug delivery strategies, amyloid diseases, or cancer therapeutics. Below are abstracts and summaries of previous capstone projects.
Academic Year 2016 - 2017
Check back later for Sarah's project abstract!
Academic Year 2015 - 2016
Check back later for Anastasia and Haya's project abstracts!
Academic Year 2014 - 2015
Bibi Murtaza's Project Abstract
Controlled release of doxorubicin from CB modified-iron oxide nanoparticles
Doxorubicin (DOX) is one of the many cytotoxic drugs currently used in cancer treatment. Its efficacy is reduced due to its severe side effects. Iron oxide (γ-Fe2O3) nanoparticles (NPs) coated with water soluble molecular containers, cucurbituril macrocycle (CB), can be used as a delivery system to load and transport DOX to cancer cells to improve its efficacy. In this study, free DOX and DOX loaded into NPCB were tested on HeLa cells for their cytotoxic effects and differences in cellular uptake. The effect of an alternating magnetic field (AMF) on the iron oxide nanoparticle-DOX delivery system was also studied. Irrespective of incubation period and dosage amount, it was evident that cellular uptake of DOX is much greater when DOX is encapsulated into NPCB. Based on our AMF studies, it can also be concluded that iron oxide delivery can be used for combining thermotherapy and chemotherapy to maximize targeted cell death.
The final, published paper can be accessed here.
Check back later for Christy and Janeen's project abstracts!
Academic Year 2013 - 2014
Katy Blumer's Project Abstract
Precise quantification of cellular uptake of cell-penetrating peptides using fluorescence-activated cell sorting and fluorescence correlation spectroscopy
Cell-penetrating peptides (CPPs) have emerged as a potentially powerful tool for drug delivery due to their ability to efficiently transport a whole host of biologically active cargoes into cells. Although concerted efforts have shed some light on the cellular internalization pathways of CPPs, quantification of CPP uptake has proved problematic. Here we describe an experimental approach that combines two powerful biophysical techniques, fluorescenceactivated cell sorting (FACS) and fluorescence correlation spectroscopy (FCS), to directly, accurately and precisely measure the cellular uptake of fluorescently-labeled molecules. This rapid and technically simple approach is highly versatile and can readily be applied to characterize all major CPP properties that normally require multiple assays, including amount taken up by cells (in moles/cell), uptake efficiency, internalization pathways, intracellular distribution, intracellular degradation and toxicity threshold. The FACS–FCS approach provides a means for quantifying any intracellular biochemical entity, whether expressed in the cell or introduced exogenously and transported across the plasma membrane.
Access the final published paper here.